Ag3.12#

The Ag3.12: Anopheles gambiae data resource contains single nucleotide polymorphism (SNP) calls, copy number variant (CNV) calls and SNP haplotypes from whole-genome sequencing of 720 mosquitoes.

More information about this release can be found in the data resource website.

This page provides an introduction to open data resources released as part of Ag3.12.

If you have any questions about this guide or how to use the data, please start a new discussion on the malariagen/vector-open-data repo on GitHub. If you find any bugs, please raise an issue.

Terms of use#

Data from this project will be made publicly available before journal publication. Unless otherwise stated, analyses of project data are ongoing and publications are in preparation by project partners, and it is not permitted to use project data for publication (including any type of communication with the general public) without prior permission from the originating partner studies.

Although malaria is generally an endemic rather than an epidemic disease, and the focus of this project is on surveillance of disease vectors rather than pathogens, our data terms of use build on MalariaGEN’s approach to data sharing, and adopt norms which have been established for rapid sharing of pathogen genomic data during disease outbreaks. The primary rationale for this approach is that malaria remains a public health emergency, where ethically appropriate and rapid sharing of genomic surveillance data can help to detect and respond to biological threats such as new forms of insecticide resistance, and to adapt malaria vector control strategies to different settings and changing circumstances.

The publication embargo for all data on this release will expire on the 4th of November 2026.

If you have any questions about the terms of use, please email support@malariagen.net

Partner studies#

1338-VO-NG-ADEDAPO (Nigeria) - Anopheles gambiae vector surveillance in Nigeria

Whole-genome sequencing and variant calling#

All samples in Ag3.12 have been sequenced individually to high coverage using Illumina technology at the Wellcome Sanger Institute. These sequence data have then been analysed to identify genetic variants such as single nucleotide polymorphisms (SNPs). After variant calling, both the samples and the variants have been through a range of quality control analyses, to ensure the data are of high quality. Both the raw sequence data and the curated variant calls are openly available for download and analysis.

For further information about the sequencing and variant calling methods used, please see the methods page.

Data hosting#

Data from Ag3.12 are hosted by several different services.

The SNP data have also been uploaded to Google Cloud, and can be analysed directly within the cloud without having to download or copy any data, including via free interactive computing services such as MyBinder and Google Colab. Further information about analysing these data in the cloud is provided in the cloud data access guide.

Sample sets#

The samples included in Ag3.12 have been organised into 1 sample set.

Each sample set corresponds to a set of mosquito specimens from a contributing study. Study details can be found in the partner studies webpages listed above.

Note: you may need to restart the kernel to use updated packages.

	sample_set	sample_count
study_id
1338-VO-NG-ADEDAPO	1338-VO-NG-ADEDAPO-VMF00268	720

Here is a more detailed breakdown of the samples contained within this sample set, summarised by country, year of collection, and species:

			taxon	arabiensis	coluzzii	gambiae
study_id	sample_set	country	year
1338-VO-NG-ADEDAPO	1338-VO-NG-ADEDAPO-VMF00268	Nigeria	2021	2	92	29
			2022	44	474	44
			2023	0	35	0

Note that there can be multiple sampling sites represented within the same sample set.

MalariaGEN vector data user guide

Ag3.12

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